Enzyme-linked immunosorbent assay (ELISA) using recombinant Fasciola cathepsin L1 for the diagnosis of human fasciolosis caused by Fasciola hepatica/gigantica hybrid type

Recombinant Fasciola cathepsin L-1 (rCatL1) was evaluated in enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of human fasciolosis Japan. Quality characteristics test were accessed by receiver operating characteristic (ROC) analysis, with sera from patients (n = 10), no evidence parasitic infections 29), and other helminth 119). Both sensitivity specificity achieved 100% control samples. To performance an authentic situation, 311 serum samples, which had been sent to our laboratory diagnosis January 2018 February 2019, re-assessed using rCatL1 ELISA. In this case, ELISA 100%, giving positive results all 7), 99.0%, three 304 non-fasciolosis samples judged positive. Careful re-examination data medical imaging these revealed that one patients, who diagnosed as having larva migrans syndrome, be infected , addition ascarid nematodes. Thus true reached 99.3% (302/304). As exhibited a highly significant likelihood ratio (152.0) negative (0.0), calculated sample data, can used routine screening definitive reference laboratories.